The effect of K(+), Ca (2+), and Mg (2+) on sperm motility in the perch, Perca fluviatilis.
Identifieur interne : 000271 ( Main/Exploration ); précédent : 000270; suivant : 000272The effect of K(+), Ca (2+), and Mg (2+) on sperm motility in the perch, Perca fluviatilis.
Auteurs : Franz Lahnsteiner [Autriche]Source :
- Fish physiology and biochemistry ; 2014.
Descripteurs français
- Wicri :
- geographic : Autriche.
English descriptors
- KwdEn :
- Animals, Austria, Calcium (pharmacology), Fisheries, Fresh Water, Magnesium (pharmacology), Male, Perches (physiology), Potassium (pharmacology), Semen (drug effects), Semen (physiology), Semen Analysis (veterinary), Sodium Chloride, Sperm Motility (drug effects), Sperm Motility (physiology), Time Factors.
- MESH :
- chemical , pharmacology : Calcium, Magnesium, Potassium.
- geographic : Austria, Sodium Chloride.
- drug effects : Semen, Sperm Motility.
- physiology : Perches, Semen, Sperm Motility.
- veterinary : Semen Analysis.
- Animals, Fisheries, Fresh Water, Male, Time Factors.
Abstract
This study investigated the effect of 0.25-5 mM K(+), Ca(2+), and Mg(2+) on sperm motility in the perch, Perca fluviatilis. In 75 mM NaCl, the used motility-activating solution, motility rate, and swimming velocity decreased within the first 4 min after activation, and the rate of locally motile sperm increased. Thereafter, the motility parameters remained constant for periods >20 min. Based on the decrease in sperm motility, two types of semen samples could be distinguished. Semen samples of type I retained a high motility rate of >65 % after 20 min, and the rate of locally motile sperm was <20 %. In semen samples of type II, the motility rate decreased to values <30 % after 20 min, and the rate of locally motile sperm exceeded >50 %. Ca(2+) and Mg(2+) concentrations of 0.25-0.5 mM had no effect on the sperm motility parameters 10 s after activation, while 0.25 mM K(+) increased the swimming velocity. K(+), Ca(2+), and Mg(2+) concentrations ≥1.5 mM had suppressive effects on the sperm motility 10 s after activation. No differences were found between the two semen types. Twenty minutes after activation, type I semen was not affected by the tested cations. On the contrary, 0.25-2.5 mM K(+), 0.25 mM Mg(2+), and 0.25-2.5 mM Ca(2+) significantly increased the sperm motility rate and/or sperm velocity of type II semen. Therefore, supplementation of saline solution with cations might stabilize the motility of perch sperm, which can be a benefit for experimental purposes and for specific handling procedures in aquaculture.
DOI: 10.1007/s10695-013-9858-7
PubMed: 24037272
Affiliations:
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Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The effect of K(+), Ca (2+), and Mg (2+) on sperm motility in the perch, Perca fluviatilis.</title><author><name sortKey="Lahnsteiner, Franz" sort="Lahnsteiner, Franz" uniqKey="Lahnsteiner F" first="Franz" last="Lahnsteiner">Franz Lahnsteiner</name><affiliation wicri:level="1"><nlm:affiliation>Federal Agency for Water Management, Institute for Water Ecology, Fisheries and Lake Research, Scharfling 18, 5310, Mondsee, Austria, Franz.Lahnsteiner@sbg.ac.at.</nlm:affiliation><country wicri:rule="url">Autriche</country><wicri:regionArea>Federal Agency for Water Management, Institute for Water Ecology, Fisheries and Lake Research, Scharfling 18, 5310, Mondsee, Austria</wicri:regionArea><wicri:noRegion>Austria</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="doi">10.1007/s10695-013-9858-7</idno><idno type="RBID">pubmed:24037272</idno><idno type="pmid">24037272</idno><idno type="wicri:Area/PubMed/Corpus">000166</idno><idno type="wicri:Area/PubMed/Curation">000166</idno><idno type="wicri:Area/PubMed/Checkpoint">000069</idno><idno type="wicri:Area/Ncbi/Merge">001118</idno><idno type="wicri:Area/Ncbi/Curation">001118</idno><idno type="wicri:Area/Ncbi/Checkpoint">001118</idno><idno type="wicri:Area/Main/Merge">000271</idno><idno type="wicri:Area/Main/Curation">000271</idno><idno type="wicri:Area/Main/Exploration">000271</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The effect of K(+), Ca (2+), and Mg (2+) on sperm motility in the perch, Perca fluviatilis.</title><author><name sortKey="Lahnsteiner, Franz" sort="Lahnsteiner, Franz" uniqKey="Lahnsteiner F" first="Franz" last="Lahnsteiner">Franz Lahnsteiner</name><affiliation wicri:level="1"><nlm:affiliation>Federal Agency for Water Management, Institute for Water Ecology, Fisheries and Lake Research, Scharfling 18, 5310, Mondsee, Austria, Franz.Lahnsteiner@sbg.ac.at.</nlm:affiliation><country wicri:rule="url">Autriche</country><wicri:regionArea>Federal Agency for Water Management, Institute for Water Ecology, Fisheries and Lake Research, Scharfling 18, 5310, Mondsee, Austria</wicri:regionArea><wicri:noRegion>Austria</wicri:noRegion></affiliation></author></analytic><series><title level="j">Fish physiology and biochemistry</title><idno type="e-ISSN">1573-5168</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Austria</term><term>Calcium (pharmacology)</term><term>Fisheries</term><term>Fresh Water</term><term>Magnesium (pharmacology)</term><term>Male</term><term>Perches (physiology)</term><term>Potassium (pharmacology)</term><term>Semen (drug effects)</term><term>Semen (physiology)</term><term>Semen Analysis (veterinary)</term><term>Sodium Chloride</term><term>Sperm Motility (drug effects)</term><term>Sperm Motility (physiology)</term><term>Time Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Calcium</term><term>Magnesium</term><term>Potassium</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Austria</term><term>Sodium Chloride</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Semen</term><term>Sperm Motility</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Perches</term><term>Semen</term><term>Sperm Motility</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Semen Analysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Fisheries</term><term>Fresh Water</term><term>Male</term><term>Time Factors</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Autriche</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study investigated the effect of 0.25-5 mM K(+), Ca(2+), and Mg(2+) on sperm motility in the perch, Perca fluviatilis. In 75 mM NaCl, the used motility-activating solution, motility rate, and swimming velocity decreased within the first 4 min after activation, and the rate of locally motile sperm increased. Thereafter, the motility parameters remained constant for periods >20 min. Based on the decrease in sperm motility, two types of semen samples could be distinguished. Semen samples of type I retained a high motility rate of >65 % after 20 min, and the rate of locally motile sperm was <20 %. In semen samples of type II, the motility rate decreased to values <30 % after 20 min, and the rate of locally motile sperm exceeded >50 %. Ca(2+) and Mg(2+) concentrations of 0.25-0.5 mM had no effect on the sperm motility parameters 10 s after activation, while 0.25 mM K(+) increased the swimming velocity. K(+), Ca(2+), and Mg(2+) concentrations ≥1.5 mM had suppressive effects on the sperm motility 10 s after activation. No differences were found between the two semen types. Twenty minutes after activation, type I semen was not affected by the tested cations. On the contrary, 0.25-2.5 mM K(+), 0.25 mM Mg(2+), and 0.25-2.5 mM Ca(2+) significantly increased the sperm motility rate and/or sperm velocity of type II semen. Therefore, supplementation of saline solution with cations might stabilize the motility of perch sperm, which can be a benefit for experimental purposes and for specific handling procedures in aquaculture.</div></front></TEI><affiliations><list><country><li>Autriche</li></country></list><tree><country name="Autriche"><noRegion><name sortKey="Lahnsteiner, Franz" sort="Lahnsteiner, Franz" uniqKey="Lahnsteiner F" first="Franz" last="Lahnsteiner">Franz Lahnsteiner</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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